InstantBlue Proteinfrbung InstantBlue ist ein gebrauchsfertiger Coomassie-Proteinfarbstoff fr Polyacrylamidgele. ISB1L. 2.4 ImageJ. . Coomassie Blue stain shows the immunoprecipitated FLAG-PAK4 and the added GST-SETD6. A single allele of HPR1 was tagged C-terminally with a Twin-Strep-3C-Protein-A tag (TSPA) in BY4741 (haploid) or BY4743 (diploid). Only proteins are stained resulting in well defined blue bands on a highly transparent background. Some premade and traditional homemade Coomassie R-250 protein stains can take three hours or more to fully stain gels. Expedeon - Instant blue - product manual Author: Expedeon protein discovery & analysis Subject: proprietary Coomassie stain that is specially formulated for ultra-fast, sensitive and safe detection of your proteins. Neobits.com is where business goes shopping.With over 5 million products from 10,000+ manufacturers, we offer the largest B2B catalog on the internet (to our knowledge).We cater to the needs of all major verticals, small to large businesses, government, education and healthcare markets, as well as consumers. The 96-well plate was coated with 2 g of His-PAK4 or BSA as control, and . Dilute this and measure the absorbance at 600 nm in a UV spectrophotometer. One gel was stained using Instant blue (Expedeon) or coomassie (see total protein stain below) and one was used to transfer the protein to a polyvinylidene fluoride (PVDF) membrane using the I-Blot transfer system (Invitrogen, UK) using programme 3 for 8.5 minutes. Description. Specifications. Proteins stained with all the InstantBlue blot are also compatible with majority spectrometry (MS) analysis. Cheap staining with just 25ml per mini-gel permits you to utilize less InstantBlue. Interstitial Fluid Biomarkers' Minimally Invasive Monitoring Using Microneedle Sensor Arrays. Its unique mechanism of action stains proteins while leaving a clear background eliminating the need to fix, wash or destain. reduced sensitivity. Limitations Leucocytes from healthy volunteers were isolated as described (Bestebroer et al . - July 2016: Acquisition of the Expedeon Holdings Ltd. Acquisition price: 15.7 M shares (subscription price EUR 1.10); EUR 1.7 M (cash) . The molecular masses were determined with marker proteins ranging from 10 to 250 kDa (Bio-Rad, Munich, Germany). 2) Washing (20 mins) 50 ml Ethanol+50 ml dd water 3) Washing with water. In all other cases, Expedeon 's liability in respect of each single incident giving rise to a claim against Expedeon, its employees or agents, shall be limited to the purchase Expedeon's InstantBlue is marketed as being a simpler, faster, and safer protein stain than Coomassie stain and other alternative reagents. Gels were stained using Instant Blue (Expedeon). While less sensitive as a colormetric method than silver, or fluorescent staining, Coomassie has undergone a significant revolution in recent years. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. InstantBlue is a prepared to-utilize Coomassie protein stain for polyacrylamide gels. instantblue expedeon - integrin signalling cell adhesion in cancer instantblue expedeon Interstitial Fluid Biomarkers' Minimally Invasive Monitoring Using Microneedle Sensor Arrays January 27, 2022 by Johanna Skin interstitial fluid (ISF) is a biofluid with information-rich biomarkers for disease diagnosis and prognosis. InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels. Samples were denatured in LDS buffer, separated on 4-12% Bis-Tris NuPage gels (Thermo Scientific) and either stained with e-Stain device from GenScript or Instant Blue (Expedeon Ltd.) Choice of mesh Samples were resolved by SDS/PAGE, and acrylamide gels were subsequently stained for protein using Instant Blue Coomasssie Blue (Expedeon) as per the manufacturer's guidelines. Instant Blue 1l Expedeon - The Scientific Publications about Aging and Longevity Home Elisa Kits Respiratory Verification Panel Tuberculosis early detection Aging Research Protein A G Biotin Biovision Lab Product Reagent Protein Assay Kit Detection Kit Enzyme Medium Serum Recombinant Protein Solution Tips cDNA Isolation Kit Gel Lentivirus Media Catalog No. The chrysalis performs a crucial role in protecting the pupa during development, while allowing the subsequent emergence of the mature butterfly. The unique patented mechanism for rapid Coomassie blue staining of protein gels begins in moments, and results are achieved within 15 minutes. InstantBlue is formulated for safe use and easy disposal. 3. List Price: The reaction was then terminated with protein sample buffer [250 mM Tris-HCl (pH 6.8), 10% SDS, 30% glycerol, 5% -mercaptoethanol, bromophenol blue] heated at 95 C for 5 min followed by SDS-PAGE and Coomassie staining (Expedeon, InstantBlue TM) or followed by in vitro methylation assay described below. Ultra-fast staining and single step procedure High sensitivity with 5 ng bands detectable $325.00 / Each of 1. Posted by Expedeon on 9th Jun 2021 InstantBlue will stain at least 40 mini . The migration tracks of the cells are indicated in blue (empty), red (PAK4 wild-type) and green (PAK4 K473R). glycerol, bromophenol blue, expedeon instant blue and Ellman's reagent were purchased from Sigma Aldrich. I'm considering Expedeon InstantBlue Coomassie protein stain for polyacrylamide gels. Expedeon 's negligence, in respect of damage to property, death or personal injury arising from any fault or defect in the materials or workmanship of the Kit, or fraud. ISB1L is the same as the 1000 ml size. The adjustment is made using the . Expedeon Protein Solutions Tel: +44 (0)1223 496500 Babraham Hall Fax: +44 (0)1223 496741 Babraham scientist@expedeon.com Cambridge CB22 3AT www.expedeon.com Innovators of Protein Technologies InstantBlue v 1.5 PLEASE READ THIS DOCUMENT CAREFULLY AS IT AFFECTS YOUR USE OF THIS EXPEDEON PRODUCT. M, molecular weight marker; Hpr1-TS, Hpr1 twin strep. Sein einzigartiger Wirkmechanismus frbt Proteine in 15 Minuten und hinterlsst einen klaren Hintergrund, so dass keine Fixierung, Wsche oder Desinfektion erforderlich ist. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Expedeon. After washing, proteins were eluted with a gradient of 0-0.5 M NaCl. Cambridge, UK, -06 January 2020: Abcam plc, a global innovator in life science reagents and tools, is pleased to announce the successful completion of the acquisition of Expedeon's Proteomics and Immunology business, which includes Innova and TGR Biosciences, as of 1st January 2020. Shown is the total protein as revealed by Instant Blue staining (left panel), as well as detection of the protein by immunoblotting using histag antibodies (right panel). Scientific TGR Biosciences August 09, 2018 16 TGR Biosciences: Commercial & Financial Blue Chip OEM customers Technology included in market leading pharma platform Established Production and R&D 12 full time staff Strong technology overlap Strong Growth SimplyBlue Safe Stain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes. ( B-D ) Stimulation of APE1-dependent (60 fmol) incision of the THF-in mononucleosome substrate (50 fmol) by increasing amounts of ( B ) wild type N-HECTD1 and ( C . 2.7 Statistical Analysis. Coomassie blue protein gel staining began in the 1960's, and it's still a fan favorite in biology labs today. The Expedeon Laboratories are manufacturing Expedeon, Expedeon Elisa, Instantblue, Instant Blue, kits and reagents distributed by Gentaur. Anti-FLAG Western blot represents input of FLAG-PAK4 transfected to HEK-293T cells (bottom panel). Entwickelt fr den sicheren Gebrauch und die . Analysis of Phaeodactylum tricornutum proteins secreted in F/2 medium under different inorganic phosphate (P i) concentrations.Coomassie blue staining revealed that two signals at approximately 130 and 100 kDa (red arrows, PtPhos2 and PtPhos1, respectively) are present only under P i-depleted conditions.A P. tricornutum cell lysate was used as positive control for the Coomassie staining. 1) Fixation (20 mins) 12 ml acetic acid + 50 ml Methanol + 15 ul Formaldehyde (37%) complete to 100 ml with distiled water. Expedeon is a world-leading expert in cutting-edge innovative reagents and services for life sciences and diagnostics, with application across workflows including: protein electrophoresis; liquid biopsy; whole genome amplification; antibody- and protein-labeling; and immunoassay development. Expedeon Innovative Products ! It is fast - in less than 15 minutes the gel is already dyed. Instant Blue the fastest and easiest protein stain on the electrophoresis market RunBlue Electrophoresis complete electrophoresis range including precast gels with unseen stability and resolution Gelfree 8100 Fractionation System for Mass Spectrometry sample preparation, One Step gel staining in 15 minutes or less. InstantBlue Protein Stain, Expedeon InstantBlue is a ready to use coomassie protein stain for polyacrylamide gels. Expedeon's InstantBlue is promoted as being a easier, quicker, and more powerful protein blot compared to Coomassie stain and other alternative reagents. Pkg of 1, 20-place rack, with removable tube rack, for use with Econo-Pac or Poly-Prep Chromatography Columns. Its unique mechanism of action stains proteins in 15 minutes, while leaving a clear background eliminating the need to fix, wash or destain. Scale bar 30 m. Immunoprecipitated samples were resolved on a 12% SDS gel and stained with Coomassie Instant Blue (Expedeon, Cambridge, UK). Samples were loaded on NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) and ran with 200 volts for 40 min. D, ELISA-based analysis of the interaction between recombinant GST-SETD6 and His-PAK4. Description. Fractions were analysed for purity using SDS-PAGE and Coomassie (Instant Blue, Expedeon) staining, and proteins of interest were identified by mass spectrometry. The protein bands were excised from the gel, . If this occurs For overview, typical data and additional information please visit: Pyrex tray, big enough to accommodate the above gel. Gels were washed with distilled water and stained with Instant Blue (Expedeon) for 1 hr. This product is manufactured by Expedeon, an Abcam company, and was previously called and was previously called InstantBlue Protein Stain (and also Optiblot Blue). Although less studied than other post-translational modifications such as phosphorylation and acetylation, the number of known methylated non-histone proteins is rapidly expanding. . InstantBlue is a ready-to-use solution stain that is specially formulated for ultra-fast (less than 15 min), sensitive (5 ng per band (BSA)) and safe detection of your proteins. What is the best Sds Page Gel Staining Solution? Esiz etki mekanizmas ile sabitleme, ykama veya boyay uzaklatrmaya gerek kalmadan proteinleri 15 dakika iinde boyamanz salar ve temiz bir arka plan brakr. Cross-linking products were resolved using 4-12% Bis-Tris NuPAGE (Invitrogen) for 5 min and briefly stained using Instant Blue (Expedeon). Both samples should now be blue. Its unique mechanism of action stains proteins in 15 minutes, leaving a clear background that eliminates the need to fix, wash or fade. Ultra fast staining - Results in 15 minutes One step procedure - No pre-washing, fixing, heating or de-staining High sensitivity - 5ng bands detectable* Clear background - High signal / noise ratio This product is for research use only and is not approved for use in humans or in clinical diagnosis. Our cutting-edge offerings include off-the-shelf . Lysine methylation of non-histone proteins has emerged as a key regulator of many cellular functions. Expression levels of His tagged proteins in CM and cell lysate fractions were analyzed by Western blotting using HisTag Bands were excised, and the proteins were reduced with 10 mM DTT for 30 min at room temperature, alkylated with 55 mM iodoacetamide for 20 min at room temperature, and digested using 13 ng/l trypsin . This stain eliminates extensive solution preparation time and expenditure. Protean II xi Cell electrophoresis system (Bio-Rad) for 200 200 1.5 mm gels, or similar. Protein gels can be stained with InstantBlue in minutes without the need to wash, fix or destain. Forml sayesinde kullanm gvenlidir; hi bir toksik madde iermez. Immunoblot Analysis. The supernatant was then incubated with Anti-TAMRA antibody (Thermo Fisher) and protein G agarose beads at 4 C overnight. We have identified the p21-activated kinase 4 (PAK4) as a new substrate for methylation by the protein lysine . Novus Biologicals InstantBlue Protein Stain. Expedeon SYGNIS Innova Biosciences C.B.S. Only the proteins are stained, resulting in extremely well defined blue bands on a highly transparent background.InstantBlue is formulated ready to use. (C) Polymerized microtubules (7.2 M tubulin) were mixed at 37C with increasing concentrations of recombinant proteins, then incubated at 4C for 45 min before . Expedeon: InstantBlue InstantBlue - While supplies last, you can purchase at a great price.. InstantBlue enables one-step staining of gels. PAK4 is classified in group II of PAKs family, all containing GTPase binding domain (GBD) and a highly conserved kinase domain 2. InstantBlue safe and ready to use Coomassie Blue protein stain for polyacrylamide gels. This product is manufactured by Expedeon, an Abcam company. Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways. Microneedle (MN) integration of sampling and instant biomarker readout hold great potential in health . The beads were washed with cell lysis buffer before being boiled with SDS loading buffer for 5 min. Eluates resulting from IgG-affinity followed by Strep-tactin (IBA) affinity purification were analyzed on 12% SDS-PAGE stained with Instant Blue (Expedeon). Antibody competition assay. Antibodies and Western blot analysis Ultrafast 15 minute staining Bands corresponding to raptor were excised and diced into small cubes (1 mm) and transferred to a clean Eppendorf per band. PAK4 is vital for embryonic development 2, 3 and is known to regulate . The radioactivity was quantified by a phosphor imager (FLA-7000; Fujifilm), and data were analyzed using the MultiGauge program (Fujifilm). Note the similar intensity of the bands at 66 kDa (Hal3) ( ) and 40 kDa (6 His-Ppz1 Cter ) ( ), indicating a 1:1 protein ratio in the eluted complex. Manufacturer: Novus Biologicals ISB1L. InstantBlue Protein Stain, Expedeon. Please contact technical@novusbio.com with questions. Other Instant products are available in stock. January 27, 2022 by Johanna. Recombinant protein yields measured by Nano-Drop2000c(ThermoScientific)were2.03mg/mland60 g/ml purified from pET28a and pET22b constructs, respectively. InstantBlue is formulated ready for use. Instant Blue RunBlue GelFree 8100 Protein Fractionation CBS: PCR workstations, electrophoresis. The strand sequences were as follows (phosphorothioate linkages are designated with *): 0S: Formulated for safe use and easy disposal, it's ready to use straight out of the . . InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels. 516938 InstantBlue Protein Stain, Expedeon Specifications UN DOT Shipping UN1805 PGIII Shipping Temp RT Storage Temp RT InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action, stains proteins in 15 minutes while leaving a clear background eliminating the need to fix, wash or destain. Figure 1. After the electrophoresis (20 mA per gel), the gels were stained with Instant Blue (Expedeon, Swavesey Cambridge, UK). InstantBlue, based on the Coomassie blue stain, provides two key benefits over other available products for faking protein bands from SDS-page gels: It provides a result in 15 minutes in comparison to a single . polyacrylamide gel electrophoresis stained with instant blue (Expedeon). 1. Description. Qty Check Availability. InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels. Stains-all was purchased from Alfa-Aesar. . Data Super quick coloring and one-venture process High responsiveness with noticeable 5 ng groups Gels can remain in the stain for weeks without concern. InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels. 2.5 Brightness and contrast adjustments. SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels. USP1 expression is up-regulated in certain breast cancers (6, 7), ovarian cancers (), colorectal cancers (), and bone cancers (), often correlated with poor prognosis (5, 7, 8).Reduction of USP1 activity via small molecules or at the level of gene expression has been shown to decrease growth of cancer cells . (B) Gels were analyzed by densitometry to quantify 6His Mn1-2 and 6His Mn1-3 proteins bound to MT (7.2 M tubulin) at 37C. Some are FDA approved or ISO13485 for IVD (In Vitro Diagnostics). Its unique mechanism of action stains proteins in 15 minutes, while leaving a clear background eliminating the need to fix, wash or destain. DNA strands were purchased from Integrated DNA Technologies. Thermo Scientific Pierce PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid. Skin interstitial fluid (ISF) is a biofluid with information-rich biomarkers for disease diagnosis and prognosis. InstantBlueTM- ready to use Coomassie protein stain for polyacrylamide gels Ordering Information CAT. This post presents a few handy tips for this essential life science pigment. The Instant Blue Gel Stain Expedeon reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. A., et al.. Membranes were incubated with Odyssey blocking buffer (Li-Cor) prior to . Our reagents are RUO, for Research use only, ISO9001 certified. Data were analyzed with ClustVis 23 to determine any clusters of individuals or species: to create heat maps, protein intensities were log10 transformed and both rows and columns are clustered using correlation distance and average linkage; . In this particular experiment, I was testing the extent of biotinylation of a purified protein on a low ug scale using streptavidin beads. Microsoft Word - 020408 Instant blue - manual . After staining with Expedeon Instant Blue dye (Abcam, Cambridge, UK), the gels were scanned with an FLA 9000 scanner (GE Healthcare, Chicago, IL, USA), and the target protein purity was assessed using ImageQuant TL v.7.0 software (GE Healthcare, Chicago, IL, USA). The empty pupal exoskeleton is also commonly referred to itself as the chrysalis, and we use this interchangeably with chrysalis exoskeleton. In Cell Reports on 26 April 2022 by Wang, L., Moreira, . Out on the market is stains that are called such as EZ- blue that stain the gel in a very quick time of 1 hour and can be destained with purified water. Expedeon product code ISB1L was previously called InstantBlue Protein Stain 1 L and is the same as the 1000 ml size of this product. 2. Download : Download high-res image (120KB) Overview. NO. Neobits.com: Buy Expedeon - BFU05L - BradfordUltra - 500mL. Its unique mechanism of action stains proteins in 15 minutes, while leaving a clear background eliminating the need to fix, wash or destain. This is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. P21-activated kinase 4 (PAK4) belongs to serine/threonine kinases family which is targeted by Cdc42 and Rac, members of small GTPases family 1. Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis. USP1 is a promising target for the treatment of several types of cancer. Instant Blue (Expedeon) or traditional Coomassie staining and destaining solutions. To buy Expedeon, Expedeon Elisa, Instantblue, Instant Blue, laboratory tools . InstantBlue poliakrilamid jeller iin kullanma hazr Coomassie protein boyasdr. Its interesting instrument of activity stains proteins quickly and leaves an unmistakable foundation, taking out the requirement for fixing, washing or destacking. DESCRIPTION ISB1L InstantBlueTMProtein Stain www.expedeon.com info@expedeon.com InstantBlueTM (Expedeon) SimplyBlueTM (Invitrogen) GelCode BlueTM (Pierce) Home-made (Anyone) The unique patented mechanism for rapid Coomassie blue staining of protein gels begins in moments, and results are achieved within 15 minutes. The dye has a formula based on Coomas and is highly sensitive. The gels were stained by Instant Blue (Expedeon) and dried using a gel dryer. Gels can remain in the stain for weeks without concern. Several Successful Products and Services Launched in 2016, Add to cart. Proteins were visualized by Instant blue stain (Expedeon). Its unique mechanism of action stains proteins in 15 minutes, while leaving a clear background eliminating the need to fix, wash or destain. So if you want fast results, please look. Brightness and contrast adjustments were made using the automatic function of the program which oversaturates the pixels outside an automatically selected grayscale range. 2.2 Labeling and Purification of Synthetic DNA Substrates 2.2.1 Equipment. Gels can remain in stain for weeks without concern. InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels. ( d ) The indicated fractions (1 mL) in panel C were collected; 10 L of each was subjected to 10% SDS-PAGE analysis and stained with Instant Blue (Expedeon). InstantBlue is a ready-to-use Coomassie protein-dye for polyacrylamide gels. You would need to experiment with the dilution The reduction of background . Antibodies, western . (Expedeon, InstantBlue) or autoradiography. 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