alexa fluor 647 equivalent

N-glycans are essential for SARS-CoV-2 function in pseudovirus model. Compare Product No. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) preadsorbed (ab150063) Reviews (4) Specific References (17) Description: 1.0 EU per 1 g of the protein by the LAL method. Quad band catalog set for Multi LED light engines like Dextran Specifications: Label (Ex/Em): Alexa Fluor 647 (650/668) Size: 10,000 MW. 2 g/lane of Recombinant Human CD19 Fc Chimera Alexa Fluor 647 Protein (Catalog # AFR9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by . Alexa Fluor 647 | C41H59N3O17PS4+ | CID 102227060 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological . Find quality proteins, antibodies, ELISA kits, laboratory reagents, and tools. Alexa Fluor 647 Technical Information. Alexa Fluor 647 dye. Anti-A2B5 Antibody, clone A2B5-105 Antibody, Alexa Fluor 647 Conjugate. When viewed under an epifluorescence microscope (Y5 filter cube: Excitation: 590-650 . 1A and table S1). Home / Spike / Recombinant SARS-CoV-2 S GCN4-IZ Alexa Fluor 647 Protein. When viewed under an epifluorescence microscope (Y5 filter cube: Excitation: 590-650 . Measured by flow cytometry for its ability to bind anti-human B7-2/CD86 Antibody conjugated beads. The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature . Product Specifications. 2 Images. With the wide range of Invitrogen Alexa Fluor dyes there is something for every filter and many options for multiplex detection. 0 Items; . They are brighter than Cy5 in aqueous mounting media. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Highly specific and rigorously validated in-house, Osteopontin/SPP1 (E4O2F) Rabbit Monoclonal Antibody (CST #27927) is ready to ship. Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): . These sites are almost fully occupied by a range of high-mannose and complex carbohydrate structures . The dye is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 647 (Alexa Fluor 647 analog) is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. For much larger or smaller proteins, select a gel . Equivalent data for each copy in the octamer is shown in Figures S2A-S2C. 336-0015 is the same as the 1 mg size. Probes with high fluorescence quantum yield and high . Concentration. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. Alexa Fluor DyesAcross the Spectrum. Recombinant SARS-CoV-2 Spike RBD Alexa Fluor 647 Protein Summary. The . Alexa Fluor 647 Dye is part of the BD red family of dyes. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. BP Fluor 647 acid. Alexa Fluor 647 anti-EGFR Antibody. A significant advantage to using long wavelength dyes such as Cy5 or BP Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. Validated for WB, IP, IF, IF. Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 647 Conjugate) #4414 Anti-Rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #79408 NOTE : When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc. The dye is water soluble and pH-insensitive from pH 4 to pH 10. Monoclonal Antibody for studying osteopontin mouse. BP Fluor 647 acid (Alexa Fluor 647 acid equivalent) is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. Alexa Fluor 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. AF647 has an excitation peak at 650 nm and an emission peak at 668 nm, and is spectrally similar to Cy5 (GE Healthcare), iFluor 647 (ATT Bioquest), APC and DyLight 650 . Perform secondary staining when using biotinylated antibodies by resuspending all wells with 50 l streptavidin staining mix. Prepare streptavidin staining mix at a final concentration of 1 g/ml streptavidin Alexa Fluor 647 in FACS buffer. This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link Rapid Alexa Fluor 647 Labeling Kit. Alexa Fluor 647 anti-GFAP Antibody. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation. Upon receipt, store it immediately at the temperature recommended below. BP Fluor 647 (Alexa Fluor 647 analog) is a water-soluble, pH-insensitive from pH 4 to pH 10 bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. BioGel P-30, or equivalent. Alexa Fluor 647 (Ex-Max 650 nm/Em-Max 668 nm): conjugates are highly photostable and remain fluorescent over a broad pH range. Source. Premounted in a BX3/IX3 filter cube (U-FF, U-M710) For Olympus BX3 and IX3 models for 25mm filters (91038) Qty in Stock: 1. ), please refer to the dye product page for the recommended protocol. Submit a product review for Human FCRL5 Alexa Fluor 647-conjugated Antibody FAB20872R-100UG. Members of the family are the leading causes of mosquito-borne viral disease worldwide. The compact, powerful, and affordable CellInsight CX5 High-Content Screening (HCS) Platform is designed for researchers wanting higher-throughput screening. BP Fluor 647 acid BP Fluor 647 acid (Alexa Fluor 647 acid equivalent) is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. Recently my lab has run into an issue when using Alexa Fluor 647 on mouse brain tissue (PFA fixed/frozen IHC). High Manufacturing Standards of Molecular Probes Dextrans. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated FcRn (E6D1S) Rabbit mAb #88175. The concentration of Recombinant Human Glypican 3 His-tag Alexa Fluor 647 (Catalog # AFR2119) that produces 50% of the binding response is .500-10. ng/mL. 7. Alexa Fluor 647 Dye is part of the BD red family of dyes. Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) Because of its broad absorption range and the fact that its emission spectrum is equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC. Alexa Fluor 532 B3 PerCP B8 PerCP/Cy5.5, BB700 B9 PerCP-eFluor 710, PerCP-Vio 700 B10 Yellow Green Excited Fluors PE YG1 PE/Dazzle 594, PE-CF594, PE-TexasRed, PE-eFluor 610 YG3 PE-Alexa Fluor 610 YG4 PE/Cy5 YG5 PE-Cy5.5, PE-AlexaFluor 700 YG7 PE/Cy7, PE-Vio 770 YG9 Red Excited Fluors APC R1 Alexa Fluor 647, Vio 667, sVio 667, efluor660 R2 APC . The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. We offer more than 50 fluorescent and biotinylated dextran conjugates in several . Recently my lab has run into an issue when using Alexa Fluor 647 on mouse brain tissue (PFA fixed/frozen IHC). Alexa Fluor 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. BP Fluor 647 (Alexa Fluor 647 equivalent; AF647) is a bright green-fluorescent dye optimal for use with the 633, 650 nm Argon laser. BP Fluor 647 647 is used for protein and . This series of superior fluorophores: Spans the near-UV, visible and near-IR spectrum ( Figure 1, Table 1 ). Hide. Are compatible with all common excitation sources ( Table 2) and . Product Usage Information Recombinant SARS-CoV-2 S GCN4-IZ Alexa Fluor 647 Protein . BP Fluor 647 Amine. rat anti-Ctip2 1/600 (Abcam) or rabbit anti-Satb2 (Abcam) in combination with the Alexa Fluor 488-, 555-, and 647-conjugated mouse/rat/rabbit secondary antibodies (Abcam; 1/400). Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass . Charge: Anionic. 337-0005 is the same as the 100 g size. A-431 cells were stained intracellularly with anti-EGFR (clone A19002A) Alexa Fluor 647 (filled histogram) or mouse IgG1, isotype control . Clonality Application Species Reactivity Citations SDS; MAB312-AF647: A2B5-105, monoclonal: FACS, ICC: Expand. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is water soluble and pH-insensitive from pH 4 to pH 10. . While dengue virus is the most prevalent, the recent Zika virus outbreak in the Americas triggered a WHO public health emergency, and yellow fever and West Nile viruses (WNV) continue to cause regional epidemics. Activity. Goat Anti-Mouse IgG H&L (Alexa Fluor 647) preadsorbed. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Invitrogen Alexa Fluor 647 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. Both primary and secondary antibodies were diluted in 2% . View our other mammalian cell tracking products. The dye is water soluble and pH-insensitive from pH 4 to pH 10. Calculate the amount of the staining mix as described in step 1. Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F . The concentration of Recombinant Human B72/CD86 Fc Chimera Alexa Fluor 647 (Catalog # AFR7625) that produces 50% of the binding response is 2.50-25. ng/mL. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Download scientific diagram | Recombinant SARS-CoV-2 RBD protein binds to human ACE2 and facilitates pseudoviral infectivity. Catalog # Availability Size / Price Qty; AFR10796-020: Add to Cart. 336-0030 is the same as the 3 x 10 ug size. . B.1.1.7 Alpha Variant (UK) His-tag. Fixable: Fixable via free amines. Mouse myeloma cell line, NS0-derived human Glypican 3 protein . Given the severity of the COVID-19 outbreak, Chroma's top priority remains the health and well-being of our employees, customers and greater communities. Alexa Fluor 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. HeNe 640 Red APCa Alexa Fluor 647 Far Red Alexa Fluor 700 Infrared BD APC-H7 APC-Cy7 Solid Stateb 405 Green BD Horizon V500 AmCyan Blue BD Horizon V450 Pacific Blue BD Influx cell sorter Solid State 488 Green FITC Alexa Fluor 488 Yellow PE Orange PE-Texas Red Red PE-Cy5 PerCP-Cy5.5 Infrared PE-Cy7 Solid State 532 or 561 Yellow PE . AFR10796 Submit a Review Datasheet. 336-0010 is the same as the 3 x 100 ug size. C57BL/6 mouse frozen cerebellum section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor 647 under optimal conditions. 336-0005 is the same as the 100 g size. Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein. R&D Systems is a global resource for cell biology. (A) Schematic of the bicistronic mRNA (3,537 bp) for the production of . Stability & Storage: . Our analyses focused on five N-linked glycans that are located proximal to either the S1-S2 proteolysis site (N61, N603, N657, and N616) or S2 cleavage site (N603 and N801) of the Spike protein (Fig. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor 647 under optimal conditions. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and . Match Criteria: Product Name. Alexa Fluor 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor 647 dye. Endotoxin Level. This Cell Signaling Technology antibody is conjugated to Alexa Fluor 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. Download : Download high-res . Nanog and GFP fluorescence were quantified in terms of molecules of equivalent soluble fluorophore (MESF) units using Quantum Alexa Fluor 488 and 647 MESF calibration beads (Bangs Laboratories, Fishers, IN). A significant advantage to using long wavelength dyes such as Cy5 or BP Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in . Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass . . The product is shipped with dry ice or equivalent. The dye has 4 sulfonate groups make it high water soluble and less aggregation in the aqueous solution. 8. Given the severity of the COVID-19 outbreak, Chroma's top priority remains the health and well-being of our employees, customers and greater communities. Application: ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P. Reactivity: Mouse. Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA, Proto-oncogene c-ErbB-1. . The NHS ester (or succinimidyl ester) of Alexa Fluor 647 is the most popular tool for conjugating this dye to a protein or antibody. Alexa Fluor 647 (AF647, Alexa 647) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 660/20 nm bandpass filter. Purity >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. 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With 2.5 g/mL of GFAP ( clone 2E1.E9 ) Alexa Fluor 647 647 is used for and! Was purified by affinity chromatography and conjugated with Alexa Fluor 647 under optimal conditions Biosciences Reference!